Hepatitis B virus markers and apolipoprotein H measure in villus tissues of pregnant womer with hepatitis B virus positive antigen

doi:10.3877/cma.j.issn.2095-3259.2020.04.010

Abstract

Abstract:

Objective

To investigate the relationship between the expression of Apolipoprotein H (Apoh) in the first trimester villi tissues of pregnant women with HBsAg positive and hepatitis B virus (HBV) intrauterine infection.

Methods

Villi tissues and blood sample of 41 HBsAg positive pregnant women during the first trimester were selected from January 2017 to May 2018. HBV DNA load in villi tissues were quantified by quantitative reverse transcription polymerase chain reaction (qRT-PCR). HBV DNA and hepatitis B markers in the blood of pregnant women were tested at admission, glial cells missing 1. HBsAg and apolipoprotein H in villi tissues were detected by immunohistochemical method.

Results

The positive rate of HBV DNA in villi tissues was 29.27% (12/41), significantly lower than 53.66% (22/41) in the blood ( χ²=5.025, P=0.043). The positive rates of HBsAg and Apoh in villi tissues was 17.07% (7/41) and 19.51% (8/41), respectively. The expression of apolipoprotein H in villi tissue HBsAg positive group was71.4%(5/7), higher than HBsAg negative group(8.8%, 3/34) ( χ²=14.487, P=0.000). The expression of apolipoprotein H in villi tissue HBsAg positive group was also higher than HBV DNA positive group 25%(3/12) ( χ²=3.909, P=0.048)

Conclusions

HBV DNA and HBsAg can be detected in villi tissues during the first trimester in HBsAg positive pregnant women. The expression of apolipoprotein H may was closely related to villi tissue HBsAg positive.

Key words: Hepatitis B virus, Chorionic villi, Apolipoprotein H, Biomarkers, Intectious disease transmission, vertical

Suhua Wang, Feng Chen, Yanjie Li, Miao Wang, Lu Cui, Shangrong Fan. Hepatitis B virus markers and apolipoprotein H measure in villus tissues of pregnant womer with hepatitis B virus positive antigen[J]. Chinese Journal of Obstetric Emergency(Electronic Edition), 2020, 09(04): 236-241.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhckjjdzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-3259.2020.04.010

https://zhckjjdzzz.cma-cmc.com.cn/EN/Y2020/V09/I04/236

Figures/Tables 4

References 21

[1]	Ott JJ, Stevens GA, Groeger J, et al. Global epidemiology of hepatitis B virus infection: new estimates of age-specific HBsAg seroprevalence and endemicity[J]. Vaccine, 2012，30(12):2212-2219. URL
[2]	Lozano R, Naghavi M, Foreman K, et al. Global and regional mortality from 235 cause of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010[J]. Lancet, 2012, 380(9859):2095-2128. URL
[3]	Zhang L, Gui X, Wang B, et al. A study of immunoprophylaxis failure and risk factors of hepatitis B virus mother-to-infant transmission[J]. Eur J Pediatr，2014，173(9):1161-1168. URL
[4]	Pan CQ, Zou HB, Chen Y, et al. Cesarean section reduces perinatal transmission of hepatitis B virus infection from hepatitis B surface antigen-positive women to their infants[J]. Clin Gastroenterol Hepatol, 2013，11(10):1349-1355.
[5]	Dwivedi M, Misra SP, Misra V, et al. Seroprevalence of hepatitis B infection during pregnancy and risk of perinatal transmission[J]. Indian J Gastroenterol, 2011, 30(2):66-71.
[6]	Korolowizc KE, Li B, Huang X, et al. Liver-Targeted Toll-Like Receptor 7 Agonist Combined With Entecavir Promotes a Functional Cure in the Woodchuck Model of Hepatitis B Virus[J]. Hepatol Commun, 2019, 3(10):1296-1310.
[7]	中华医学会感染病学分会，中华医学会肝病学分会. 慢性乙型肝炎防治指南(2019年版)[J]. 中华临床感染病杂志. 2019，12(6):401-428.
[8]	彭婷婷，王淼，陈凤，等. HBsAg阳性孕妇早期流产绒毛组织中HBV标志物检测及风险因素研究[J]．中华实验和临床病毒学杂志，2019，33(2):182-186.
[9]	Wang Y, Zhou H, Zhang L, et al. Prevalence of chronic hepatitis B and status of HBV care among rural women who planned to conceive in China[J]. Sci Rep, 2017，(1):12090.
[10]	Zheng H, Cui FQ, Wang FZ, et al. The epidemiology of hepatitis B virus infection in women of reproductive age in highly endemic areas in China[J]. J Viral Hepat, 2018, 25(1):88-96.
[11]	Peng TT, Cai QE, Yang M, et al. Epidemiological trends and virological traits of hepatitis B virus Infection in pregnant women and neonates[J]. Arch Virol, 2019，164(5):1335-1341.
[12]	Chen Y, Wang L, Xu Y, et al. Role of maternal viremia and placental infection in hepatitis B virus intrauterine transmission[J]. Microbes Infect, 2013，15(5):409-415. URL
[13]	Xu DZ, Yan YP, Zou S, et al. Role of placental tissues in the intrauterine transmission of hepatitis B virus[J]. Am J Obstet Gynecol, 2001，185 (4):981-987.
[14]	Zhang Z, Li A, Xiao XM. Risk factors for intrauterine infection with hepatitis B virus[J]. Int J Gynecol Obstet, 2014, 125(2): 158-161. URL
[15]	Guo Z, Shi XH, Feng YL, et al. Risk factors of HBV intrauterine transmission among HBsAg-positive pregnant women[J]. J Viral Hepat, 2013, 20(5): 317-321.
[16]	Yin YZ, Wu LL, Zhang J, et al. Identification of risk factors associated with immunoprophylaxis failure to prevent the vertical transmission of hepatitis B virus[J]. J infect Infection, 2013, 66(5): 447-452.
[17]	Stefas I, Tigrett S, Dubois G, et al. Interactions between Hepatitis C Virus and the Human Apolipoprotein H Acute Phase Protein: A Tool for a Sensitive Detection of the Virus[J]. PLoS One. 2015，10(10):e0140900.
[18]	Wang M, Kong X, Xie Y, et al. Role of TLR？4 in anti？β2？glycoprotein I？induced activation of peritoneal macrophages and vascular endothelial cells in mice[J]. Mol Med Rep, 2019, 19(5):4353-4363.
[19]	Liu YM, Zhang WY, Wang ZF, et al. High Expression of Beta2-Glycoprotein I is Associated Significantly With the Earliest Stages of Hepatitis B Virus Infection[J]. J Med Virol, 2014, 86(8): 1296-1306. URL
[20]	Stefas I, Rucheton M, D′Angeac AD, et al. Hepatitis B virus Dane particles bind to human plasma Apolipoprotein H[J]. Hepatology, 2001，33(1):207-217.
[21]	Chamley LW, Allen JL, Johnson PM. Synthesis of beta2 glycoprotein1 by the human placenta[J]. Placenta, 1997，18(5-6):403-410.

组别	例数	HBsAg(IU/ml)	HBsAb(IU/ml)	HBeAg(S/CO)	HBeAb(S/CO)	HBcAb(S/CO)	HBV DNA(IU/ml)
HBV DNA阳性	12	33 615.6(4576.7, 54 059.5)	0.38(0, 1.25)	1054.5(0.43, 1488.9)	25.0(0.355, 56.36)	8.93(6.74, 9.62)	3.06×10⁷(1.54×10^6, 6.0×10⁷)
HBV DNA阴性	29	2403.5(673.6, 5084.8)	0.41(0, 0.76)	0.25(0.23, 0.29)	0.02(0.01, 0.105)	9.42(8.62, 9.88)	100(100, 876.5)
Z值		-3.037	-0.146	-3.439	-3.905	-1.075	-4.281
P值		0.002	0.884	0.001	0.000	0.283	0.000

组别	例数	HBsAg(IU/ml)	HBsAb(IU/ml)	HBeAg(S/CO)	HBeAb(S/CO)	HBcAb(S/CO)	HBV DNA(IU/ml)
HBV DNA阳性	12	33 615.6(4576.7, 54 059.5)	0.38(0, 1.25)	1054.5(0.43, 1488.9)	25.0(0.355, 56.36)	8.93(6.74, 9.62)	3.06×10⁷(1.54×10^6, 6.0×10⁷)
HBV DNA阴性	29	2403.5(673.6, 5084.8)	0.41(0, 0.76)	0.25(0.23, 0.29)	0.02(0.01, 0.105)	9.42(8.62, 9.88)	100(100, 876.5)
Z值		-3.037	-0.146	-3.439	-3.905	-1.075	-4.281
P值		0.002	0.884	0.001	0.000	0.283	0.000

组别	例数	HBsAg(IU/ml)	HBsAb(IU/ml)	HBeAg(S/CO)	HBeAb(S/CO)	HBcAb(S/CO)	HBV DNA(IU/ml)
HBsAg阳性	7	6721.6(1153.1, 32872.0)	0.30(0.00, 1.42)	0.23(0.22, 178.5)	0.10(0.01, 5.54)	8.66(8.32, 9.87)	6230(100, 1.75×10⁷)
HBsAg阴性	34	3012.2(588.1, 9061.4)	0.42(0.00, 0.74)	0.26(0.24, 14.5)	0.035(0.01, 1.25)	9.37(8.22, 9.83)	107.5(100, 20585.00)
Z值		-1.039	-0.053	-1.126	-0.141	-0.641	-1.095
P值		0.314	0.959	0.267	0.906	0.530	0.314

组别	例数	HBsAg(IU/ml)	HBsAb(IU/ml)	HBeAg(S/CO)	HBeAb(S/CO)	HBcAb(S/CO)	HBV DNA(IU/ml)
HBsAg阳性	7	6721.6(1153.1, 32872.0)	0.30(0.00, 1.42)	0.23(0.22, 178.5)	0.10(0.01, 5.54)	8.66(8.32, 9.87)	6230(100, 1.75×10⁷)
HBsAg阴性	34	3012.2(588.1, 9061.4)	0.42(0.00, 0.74)	0.26(0.24, 14.5)	0.035(0.01, 1.25)	9.37(8.22, 9.83)	107.5(100, 20585.00)
Z值		-1.039	-0.053	-1.126	-0.141	-0.641	-1.095
P值		0.314	0.959	0.267	0.906	0.530	0.314

绒毛组织	例数	Apoh阳性数	阳性率(%)
HBV DNA阳性	12	3	25.0^a
HBV DNA阴性	29	5	17.2
HBsAg阳性	7	5	71.4^b，c
HBsAg阴性	34	3	8.8

Please choose a citation manager

Content to export