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Chinese Journal of Obstetric Emergency(Electronic Edition) ›› 2022, Vol. 11 ›› Issue (01): 46-52. doi: 10.3877/cma.j.issn.2095-3259.2022.01.010

• Experimental Research • Previous Articles     Next Articles

Selection of internal references gene for studying trophoblast syncytialization

Wanshan Zheng1, Yin Yin2, Zhaowei Tu3, Bin Cao4,()   

  1. 1. Department Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong Province Key Laboratory of Major Obstetric Diseases, Guangzhou Medical Center for Critical Pregnant Women, Guangzhou 510150, China; Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine Xiamen University, Xiamen 361102, China
    2. Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
    3. Department Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, Guangdong Province Key Laboratory of Major Obstetric Diseases, Guangzhou Medical Center for Critical Pregnant Women, Guangzhou 510150, China
    4. Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine Xiamen University, Xiamen 361102, China
  • Received:2021-08-26 Online:2022-02-18 Published:2022-04-28
  • Contact: Bin Cao

Abstract:

Objective

To screen and identify reference genes suitable for studying on trophoblast cells.

Methods

The stability of 11 candidate reference genes were compared using BeWo and human trophoblast stem cells (hTSCs). Three software programs, GeNorm, NormFinder, and BestKeeper, were used to determine the most suitable internal reference genes for the study of trophoblast cell syncytialization.

Results

TBP and GRPS13 were stable reference genes, while GAPDH and β-actin expression levels were not stable during trophoblast cell syncytialization.

Conclusions

TBP and RPS13 were recommended as the preferred internal reference genes for trophoblast syncytialization studies using RT-PCR.

Key words: Trophoblasts, Cell line, Cell fusion, Polymerase chain reaction

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