缩宫素对大鼠产后出血肾脏缺血再灌注损伤病理改变和抗氧化机制的影响

doi:10.3877/cma.j.issn.2095-3259.2012.02.015

目的

探讨缩宫素对妊娠大鼠产后出血致肾脏缺血再灌注损伤的保护作用及其可能的机制。

方法

将妊娠足月Wistar大鼠随机(随机数字表法)分为3组，每组30只。即缺血再灌注组、缩宫素处理组和对照组。建立大鼠产后失血性休克再灌注模型，观察肾脏的病理组织学改变并检测血中肌酐(Cr)、血尿素氮(blood urea nitrogen，BUN)的水平和肾组织中丙二醛(MDA)、过氧化物歧化酶(SOD)及谷胱甘肽－过氧化物酶(GSH-PX)3种抗氧化损伤指标的活性。各组间指标的比较采用SNK法分析。

结果

(1)缺血再灌注组肾脏间质灶状出血，肾小球萎缩，肾小管肿胀；缩宫素处理组肾脏间质出血明显减轻，肾小球及肾小管结构基本正常。(2)缺血再灌注组Cr(151.58±14.31)μmol/L，BUN(48.73±2.48)mmol/L；缩宫素处理组Cr(83.43±4.67)μmol/L，BUN(37.57±0.50)mmol/L；两组比较差异有统计学意义(q＝3.1551，3.1526；P<0.05)。(3)缺血再灌注组，肾组织中MDA(0.88±0.13)nmol/mg，SOD(28.54±1.31)U/mg，GSH-PX(2.94±0.24)U/mg；而缩宫素处理组MDA(0.12±0.56)nmol/mg，SOD(52.23±2.29) U/mg，GSH-PX(5.83±0.14)U/mg，两组比较差异有统计学意义(q＝3.8293，3.1232，2.9756；P<0.05)。

结论

缩宫素对产后失血性休克大鼠再灌注后的肾脏病理改变和肾功能具有一定的保护作用，其机制可能与抑制脂质过氧化反应有关。

关键词: 缩宫素, 产后出血, Wistar大鼠, 肾, 再灌注损伤

Objective

To evaluate the protective role of oxytocin(OT) on protecting the kidney against ischemia/reperfusion injury and its mechanisms in postpartum hemorrhage rats.

Methods

Wistar rats were randomly divided into 3 groups, which were ischemia/reperfusion group, oxytocin treatment group and control group, respectively. Each group included 30 rats. Postpartum hemorrhage shock rat model was established, and histological changes of kidney samples were observed. Malondialdehyde (MDA), superoxide dismutase (SOD), glutathione-peroxidase (GSH-PX) of kidney samples were measured. Creatinine (Cr), blood urea nitrogen (BUN) of blood samples were assayed. The data were analyzed using SNK method.

Results

(1) In ischemia/reperfusion group, kidneys were local hemorrhage in renal interstitial substance, glomerular atrophy and renal tubule edema. In oxytocin treatment group, local hemorrhage were relieved significantly, and the structure of glomerulus and renal tubule were normal. (2) In ischemia/reperfusion group and oxytocin treatment group, Cr were 151.58±14.31 μmol/L and 83.43±4.67 μmol/L, respectively; and BUN were respectively 48.73±2.48 mmol/L and 37.57±0.50 mmol/L. Compared with two groups, q=3.1551 and 3.1526, p value were all less than 0.05. (3) Compared with ischemia/reperfusion group and oxytocin treatment group, MDA were 0.88±0.13 nmol/mg and 0.12±0.56 nmol/mg; SOD were 28.54±1.31 U/mg and 52.23±2.29 U/mg; and GSH-PX were 2.94±0.24 U/mg and 5.83±0.14 U/mg, respectively. Compared with two groups, q=3.8293, 3.1232 and 2.9756; p value were all less than 0.05.

Conclusions

For renal pathological changes and renal function, oxytocin has protective effect on renal ischemia-reperfusion injury in postpartum hemorrhage shock rats. Inhibiting peroxidation may be their mechanisms.

Key words: Oxytocin, Postpartum hemorrhage, Rats, wistar, Kidney, Reperfusion injury

图1 对照组大鼠肾脏组织光镜观察图像　显示肾小球和肾小管结构正常(HE　×400)

图2 缺血再灌注组大鼠肾脏组织光镜观察图像　显示肾脏间质灶状出血，肾小球萎缩，肾小管肿胀(HE　×400)

图3 缩宫素处理组大鼠肾脏组织光镜观察图像　显示肾脏间质出血明显减轻，肾小球及肾小管结构基本正常(HE　×400)

表1 各组大鼠血清Cr和BUN检测值的比较

组别	鼠数(只)	Cr (μmol/L)	BUN(mmol/L)
缺血再灌注组	30	151.58±14.31^ab	48.73±2.48^de
缩宫素处理组	30	83.43±4.67^c	37.57±0.50^f
对照组	30	68.03±3.70	15.23±1.50
F值	?	44.109	23.312
P值	?	0.000	0.000

表1 各组大鼠血清Cr和BUN检测值的比较

组别	鼠数(只)	Cr (μmol/L)	BUN(mmol/L)
缺血再灌注组	30	151.58±14.31^ab	48.73±2.48^de
缩宫素处理组	30	83.43±4.67^c	37.57±0.50^f
对照组	30	68.03±3.70	15.23±1.50
F值	?	44.109	23.312
P值	?	0.000	0.000

表2 各组大鼠肾组织MDA、SOD及GSH-Px检验值的比较

组别	鼠数(只)	MDA(nmol/mg)	SOD(U/mg)	GSH-PX(U/mg)
缺血再灌注组	30	0.88±0.13^ab	28.54±1.31^cd	2.94±0.24^fg
缩宫素处理组	30	0.12±0.06	52.23±2.29^e	5.83±0.14^h
对照组	30	0.22±0.13	74.42±2.65	9.09±2.39
F值	?	37.347	52.721	20.218
P值	?	0.000	0.000	0.000

表2 各组大鼠肾组织MDA、SOD及GSH-Px检验值的比较

组别	鼠数(只)	MDA(nmol/mg)	SOD(U/mg)	GSH-PX(U/mg)
缺血再灌注组	30	0.88±0.13^ab	28.54±1.31^cd	2.94±0.24^fg
缩宫素处理组	30	0.12±0.06	52.23±2.29^e	5.83±0.14^h
对照组	30	0.22±0.13	74.42±2.65	9.09±2.39
F值	?	37.347	52.721	20.218
P值	?	0.000	0.000	0.000

[1]	王晨虹，龚时鹏，余艳红，等. 限制性液体复苏对失血性休克孕兔肾功能的影响．医学临床研究，2007,24(6):887-892.
[2]	Pournajafi-Nazarloo H, Perry A, Partoo L, et al. Neonatal oxytocin treatment modulates oxytocin receptor, atrial natriuretic peptide, nitric oxide synthase and estrogen receptor mRNAs expression in rat heart. Peptides, 2007, 28(6):1170-1177.
[3]	Işeri SO, Gedik IE, Erzik C, et al. Oxytocin ameliorates skin damage and oxidant gastric injury in rats with thermal trauma. Burns, 2008, 34(3):361-369.
[4]	Solomonov E, Hirsh M, Yahiya A, et al. The effect of vigorous fluid resuscitation in uncontrolled hemorrhagic shock after massive splenic injury. Crit Care Med, 2000, 28(3):749-754.
[5]	王汉民，吴雄飞，谭华，等．川芎嗪对缺血/再灌注损伤大鼠肾脏细胞凋亡及Bcl-2和Bax表达的影响．第四军医大学学报，2006,27(20):1884-1887.
[6]	尹明华，徐晓虹，姜科声. 灯盏花素对缺血再灌注诱导小鼠学习记忆损伤的保护作用．中国药学杂志，2007,42(7):497-500.
[7]	Oksuz H, Bulbuloglu E, Senoglu N, et al. Re-protective effects of pre- and post-laparoscopy conditioning, zinc, pentoxifylline, and N-acetylcysteine in an animal model of laparoscopy-induced ischemia/reperfusion injury of the kidney. Ren Fail, 2009, 31(4):297-302.
[8]	Salehipour M, Monabbati A, Salahi H, et al. Protective effect of parenteral vitamin E on ischemia-reperfusion injury of rabbit kidney. Urology, 2010, 75(4):858-861.
[9]	Sankaramanivel S, Rajaram A, Rajaram R. Zinc protects human peripheral blood lymphocytes from Cr(III)(phenanthroline) 3-induced apoptosis. Toxicol Appl Pharmacol, 2010, 243(3):405-419.
[10]	TuAgˇG1tepe H, Sener G, Biyikli NK, et al. The protective effect of oxytocin on renal ischemia/reperfusion injury in rats. Regul Pept, 2007,140(3):101-108.
[11]	姚丹丹，唐高兴. 银杏叶提取物对肾缺血再灌注损伤的保护作用. 实用医学杂志，2008, 24(9):1495-1496.
[12]	李俊龙，孙则禹，陈明，等. 红景天甙对大鼠肾脏缺血再灌注损伤的保护作用. 现代泌尿外科杂志，2008, 13(3):164-166.
[13]	蔡虹静，苏庆国，徐芳，等. 黄芪注射液和维生素E联合使用时对大鼠肾缺血再灌注损伤的影响. 中医杂志，2011, 52(17):1496-1499.
[14]	贾昌盛，孙建军，李美德，等．苦参素降低大鼠肾脏缺血-再灌注损伤. 基础医学与临床，2012,32(8):943-947.
[15]	Zhou JF, Chen JX, Shen HC, et al. Abnormal reactions of free radicals and oxidative damages in the bodies of patients with chronic glomerulonephritis. Biomed Environ Sci, 2002, 15(3):233-244.
[16]	Masztalerz M, Wlodarczyk Z, Czuczejko J, et al. Superoxide anion as a marker of ischemia-reperfusion injury of the transplanted kidney. Transplant Proc, 2006, 38(1):46-48.
[17]	杨舜，裴凌. 异丙酚和氯胺酮减轻大鼠肾缺血再灌注损伤. 中国病理生理杂志，2010,26(1):177-180.
[18]	李祖成，李淑翠，亢泽春，等. 硫酸镁对小鼠肾缺血再灌注损伤保护作用．中国公共卫生，2012,28(8):1059-1061.

[1]	张舒沁, 陈练. 产后宫腔内妊娠物残留的诊断和临床处理[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(05): 493-497.
[2]	李霞林, 贺芳. 产后出血风险评估和早期预警系统[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(05): 498-503.
[3]	石海波, 赵旭东, 王聪, 曲巍. 气肿性肾盂肾炎、气肿性膀胱炎并脓毒性休克一例报道并文献复习[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(06): 644-647.
[4]	曹彬, 王强, 卢扬柏, 黄红星, 黄亚强, 龙永富, 钟睿, 李灿永, 罗刚. 单孔经皮肾镜和腹腔镜处理肾囊肿的术式对比研究[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(06): 566-571.
[5]	熊鹰, 林敬莱, 白奇, 郭剑明, 王烁. 肾癌自动化病理诊断：AI离临床还有多远？[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(06): 535-540.
[6]	石兵, 张智, 陈金海, 唐文. 基于电磁跟踪和手术导航系统的实时超声引导下两种经皮肾盏穿刺方法的应用[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(06): 572-578.
[7]	杨柳, 陈佳, 孙雅娟, 陈娇, 谭明超, 龚明福. 抗中性粒细胞胞浆抗体相关性血管炎的胸部CT 及临床特征分析[J/OL]. 中华肺部疾病杂志(电子版), 2024, 17(05): 744-749.
[8]	孙璐, 蒋亚玲, 陈凌君. 布托啡诺对脑缺血再灌注损伤大鼠神经炎症和JAK2/STAT3信号通路的影响[J/OL]. 中华细胞与干细胞杂志(电子版), 2024, 14(06): 344-350.
[9]	周学锋, 董哲毅, 冯哲, 蔡广研, 陈香美. 糖尿病肾脏疾病中西医结合诊疗指南计划书[J/OL]. 中华肾病研究电子杂志, 2024, 13(06): 301-305.
[10]	史彬, 司远. 益气和络方联合缬沙坦治疗气阴两虚兼血瘀证IgA 肾病的疗效观察[J/OL]. 中华肾病研究电子杂志, 2024, 13(06): 306-312.
[11]	国文凯, 纪鹏程, 毕靖茹, 谢院生. IgA 肾病的十种治疗措施[J/OL]. 中华肾病研究电子杂志, 2024, 13(06): 327-333.
[12]	程柏凯, 杨光. 高胰岛素-正葡萄糖钳夹技术评估慢性肾脏病患者胰岛素抵抗的研究进展[J/OL]. 中华肾病研究电子杂志, 2024, 13(06): 334-339.
[13]	陈意志. 核磁共振钆造影剂导致的肾源性系统性纤维化[J/OL]. 中华肾病研究电子杂志, 2024, 13(06): 358-358.
[14]	沈炎, 张俊峰, 唐春芳. 预后营养指数结合血清降钙素原、胱抑素C及视黄醇结合蛋白对急性胰腺炎并发急性肾损伤的预测价值[J/OL]. 中华消化病与影像杂志(电子版), 2024, 14(06): 536-540.
[15]	颜世锐, 熊辉. 感染性心内膜炎合并急性肾损伤患者的危险因素探索及死亡风险预测[J/OL]. 中华临床医师杂志(电子版), 2024, 18(07): 618-624.

阅读次数

全文

摘要

选择文件类型/文献管理软件名称

选择包含的内容

The effect of oxytocin on renal ischemia/reperfusion injury induced the pathologic change and antioxidation mechanisms in postpartum hemorrhage rats

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

The effect of oxytocin on renal ischemia/reperfusion injury induced the pathologic change and antioxidation mechanisms in postpartum hemorrhage rats